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smart mmlv reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa smart mmlv reverse transcriptase
    Smart Mmlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 5568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+mmlv+reverse+transcriptase/SMART+MMLV+Reverse+Transcriptase/10__1016_slash_j__eti__2026__104886-65-6-10
    Average 97 stars, based on 5568 article reviews
    smart mmlv reverse transcriptase - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Generated:

    Article Title: Involvement of the African swine fever virus protein pH108R in virion morphogenesis.
    Article Snippet: .. Total cellular RNA from PAMs was then extracted utilizing TRIzol Reagent (Catalogue #15596018, Invitrogen, Carlsbad, USA), and cDNAs were generated using the SMART MMLV Reverse Transcriptase (Catalogue #639522, Takara Biotechnology, Dalian, China). .. Transcription levels of H108R, CP204L, B646L and pA104R genes were evaluated by qRT-PCR utilizing the TB Green Premix (Catalogue #RR820A, Takara Biotechnology, Dalian, China) following the manufacturer’s instructions.

    Article Title: Involvement of the African swine fever virus protein pH108R in virion morphogenesis
    Article Snippet: .. Total cellular RNA from PAMs was then extracted utilizing TRIzol Reagent (Catalogue #15596018, Invitrogen, Carlsbad, USA), and cDNAs were generated using the SMART MMLV Reverse Transcriptase (Catalogue #639522, Takara Biotechnology, Dalian, China). .. Transcription levels of H108R , CP204L , B646L and pA104R genes were evaluated by qRT-PCR utilizing the TB Green Premix (Catalogue #RR820A, Takara Biotechnology, Dalian, China) following the manufacturer's instructions.

    Reverse Transcription:

    Article Title: Involvement of the African swine fever virus protein pH108R in virion morphogenesis.
    Article Snippet: .. Total cellular RNA from PAMs was then extracted utilizing TRIzol Reagent (Catalogue #15596018, Invitrogen, Carlsbad, USA), and cDNAs were generated using the SMART MMLV Reverse Transcriptase (Catalogue #639522, Takara Biotechnology, Dalian, China). .. Transcription levels of H108R, CP204L, B646L and pA104R genes were evaluated by qRT-PCR utilizing the TB Green Premix (Catalogue #RR820A, Takara Biotechnology, Dalian, China) following the manufacturer’s instructions.

    Article Title: A Highly Conserved SNARE-Associated Protein Enhances Plant Immunity by Regulating Vesicle Trafficking.
    Article Snippet: .. Total RNA extracted from three time points was combined and mRNA was isolated from the combined total RNA. cDNA was synthesised from 1 μg of isolated mRNA using SMART III- modified oligo and SMART MMLV reverse transcriptase (Takara Bio). ..

    Article Title: Evaluation of low-density polyethylene biodegradation by Bacillus safensis BS-10L after plasma treatment under environmental stress and underlying mechanisms
    Article Snippet: Total RNA was isolated from cultured bacterial cells using an RNA extraction kit (TAKARA, Tokyo, Japan). .. Complementary DNA (cDNA) was synthesized using SMART® MMLV Reverse Transcriptase (Clontech, Mountain View, California, USA) following the manufacturer’s instructions. .. One microliter of the synthesized cDNA was used as the template for quantitative reverse transcription polymerase chain reaction (qRT-PCR). qRT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad, Hercules, California, USA) and a Real-Time PCR detection system (Bio-Rad).

    Article Title: Involvement of the African swine fever virus protein pH108R in virion morphogenesis
    Article Snippet: .. Total cellular RNA from PAMs was then extracted utilizing TRIzol Reagent (Catalogue #15596018, Invitrogen, Carlsbad, USA), and cDNAs were generated using the SMART MMLV Reverse Transcriptase (Catalogue #639522, Takara Biotechnology, Dalian, China). .. Transcription levels of H108R , CP204L , B646L and pA104R genes were evaluated by qRT-PCR utilizing the TB Green Premix (Catalogue #RR820A, Takara Biotechnology, Dalian, China) following the manufacturer's instructions.

    Article Title: A Highly Conserved SNARE ‐Associated Protein Enhances Plant Immunity by Regulating Vesicle Trafficking
    Article Snippet: .. Total RNA extracted from three time points was combined and mRNA was isolated from the combined total RNA. cDNA was synthesised from 1 μg of isolated mRNA using SMART III‐modified oligo and SMART MMLV reverse transcriptase (Takara Bio). .. The synthesised cDNA was amplified with Long Distance PCR (LD‐PCR) using Advantage 2 Polymerase Mix (Takara Bio).

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..

    Article Title: Single-cell profiling reveals FAM117A as a key regulator linking macrophage–epithelial crosstalk with the progression of lung adenocarcinoma
    Article Snippet: Total RNA was extracted from individual cell suspensions utilizing either the RNeasy Mini Kit (QIAGEN, 74106) or RNA-Bee (Tel-Test). .. The poly(A)-tailed mRNA was subsequently reverse transcribed with SMART MMLV reverse transcriptase (Takara Bio, 639524). .. PCR amplification was carried out on a BioRad real-time PCR machine utilizing the SYBR green QPCR mix (Quantabio, 95056–02 K) and gene-specific primers that span introns.

    Isolation:

    Article Title: A Highly Conserved SNARE-Associated Protein Enhances Plant Immunity by Regulating Vesicle Trafficking.
    Article Snippet: .. Total RNA extracted from three time points was combined and mRNA was isolated from the combined total RNA. cDNA was synthesised from 1 μg of isolated mRNA using SMART III- modified oligo and SMART MMLV reverse transcriptase (Takara Bio). ..

    Article Title: A Highly Conserved SNARE ‐Associated Protein Enhances Plant Immunity by Regulating Vesicle Trafficking
    Article Snippet: .. Total RNA extracted from three time points was combined and mRNA was isolated from the combined total RNA. cDNA was synthesised from 1 μg of isolated mRNA using SMART III‐modified oligo and SMART MMLV reverse transcriptase (Takara Bio). .. The synthesised cDNA was amplified with Long Distance PCR (LD‐PCR) using Advantage 2 Polymerase Mix (Takara Bio).

    Modification:

    Article Title: A Highly Conserved SNARE-Associated Protein Enhances Plant Immunity by Regulating Vesicle Trafficking.
    Article Snippet: .. Total RNA extracted from three time points was combined and mRNA was isolated from the combined total RNA. cDNA was synthesised from 1 μg of isolated mRNA using SMART III- modified oligo and SMART MMLV reverse transcriptase (Takara Bio). ..

    Synthesized:

    Article Title: Evaluation of low-density polyethylene biodegradation by Bacillus safensis BS-10L after plasma treatment under environmental stress and underlying mechanisms
    Article Snippet: Total RNA was isolated from cultured bacterial cells using an RNA extraction kit (TAKARA, Tokyo, Japan). .. Complementary DNA (cDNA) was synthesized using SMART® MMLV Reverse Transcriptase (Clontech, Mountain View, California, USA) following the manufacturer’s instructions. .. One microliter of the synthesized cDNA was used as the template for quantitative reverse transcription polymerase chain reaction (qRT-PCR). qRT-PCR was performed using iQ SYBR Green Supermix (Bio-Rad, Hercules, California, USA) and a Real-Time PCR detection system (Bio-Rad).

    Polymerase Chain Reaction:

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..

    Amplification:

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..

    Article Title: A workflow for quantifying cell quiescence in 3D spheroids
    Article Snippet: RNA extraction was performed using TRIzol Reagent (Invitrogen, MA, USA). .. RNA (200/300 ng) was reverse transcribed with SMART MMLV-Reverse Transcriptase (Takara, Japan) and subjected to PCR amplification using the SensiMix SYBRLox-ROX Kit (Meridian Bioscience, OH, USA). ..



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